Identification and characterization of suppressor mutants of spl11-mediated cell death in rice

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dc.creatorShirsekar, Gautam S.
dc.creatorVega-Sanchez, Miguel E.
dc.creatorBordeos, Alicia
dc.creatorBaraoidan, Marietta
dc.creatorSwisshelm, Andrew
dc.creatorFan, Jiangbo
dc.creatorPark, Chan Ho
dc.creatorLeung, Hei
dc.creatorWang, Guo-Liang
dc.date2014-06
dc.date2024-12-19T12:55:09Z
dc.date2024-12-19T12:55:09Z
dc.date.accessioned2026-06-27T04:13:11Z
dc.descriptionLesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe oryzae. Spl11 encodes an E3 ubiquitin ligase and is a negative regulator of PCD in rice. To study the regulation of Spl11-mediated PCD, we performed a genetic screen and identified three spl11 cell-death suppressor (sds) mutants. These suppressors were characterized for their resistance to X. oryzae pv. oryzae and M. oryzae and for their expression of defense-related genes. The suppression of the cell-death phenotypes was generally correlated with reduced expression of defense-related genes. When rice was challenged with avirulent isolates of M. oryzae, the disease phenotype was unaffected in the sds mutants, indicating that the suppression might be Spl11-mediated pathway specific and may only be involved in basal defense. In addition, we mapped one of the suppressor mutations to a 140-kb interval on the long arm of rice chromosome 1. Identification and characterization of these sds mutants should facilitate our efforts to elucidate the Spl11-mediated PCD pathway.
dc.identifierhttps://hdl.handle.net/10568/165515
dc.identifier.urihttp://hdl.handle.net/123456789/25512
dc.languageen
dc.publisherScientific Societies
dc.sourceShirsekar, Gautam S.; Vega-Sanchez, Miguel E.; Bordeos, Alicia; Baraoidan, Marietta; Swisshelm, Andrew; Fan, Jiangbo; Park, Chan Ho; Leung, Hei and Wang, Guo-Liang. 2014. Identification and characterization of suppressor mutants of spl11-mediated cell death in rice. MPMI, Volume 27 no. 6 p. 528-536
dc.subjectchromosomes
dc.subjectdisease resistance
dc.subjectenzymes
dc.subjectfungi
dc.subjectgenes
dc.subjectmagnaporthe grisea
dc.subjectmutants
dc.subjectmutations
dc.subjectpathogens
dc.subjectphenotypes
dc.subjectplant pathogenic bacteria
dc.subjectplant pathogenic fungi
dc.subjectxanthomonas oryzae pv
dc.subjectoryzae
dc.titleIdentification and characterization of suppressor mutants of spl11-mediated cell death in rice
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)