Cell size regulation and proliferation fluctuations in single-cell derived colonies

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dc.audienceInvestigador
dc.coverageC.I Tibaitatá
dc.coverageEstados Unidos
dc.creatorNieto, César
dc.creatorVargas García, César
dc.creatorPedraza, Juan Manuel
dc.creatorSingh, Abhyudai
dc.date2024-05-22T16:15:55Z
dc.date2024-05-22T16:15:55Z
dc.date2022-07-06
dc.date2022
dc.date.accessioned2026-06-27T04:38:01Z
dc.descriptionExponentially growing cells regulate their size by controlling their timing of division. Since two daughter cells are born as a result of this cell splitting, cell size regulation has a direct connection with cell proliferation dynamics. Recent models found more clues about this connection by suggesting that division occurs at a size-dependent rate. In this article, we propose a framework that couples the stochastic transient dynamics of both the cell size and the number of cells in the initial expansion of a single-cell-derived colony. We describe the population from the two most common perspectives. The first is known as Single Lineage: where only one cell is followed in each colony, and the second is Population Snapshots: where all cells in different colonies are followed. At a low number of cells, we propose a third perspective; Single Colony, where one tracks only cells with a common ancestor. We observe how the statistics of these three approaches are different at low numbers and how the Single Colony perspective tends to Population Snapshots at high numbers. Analyzing colony-to-colony fluctuations in the number of cells, we report an intriguing find: the extent of fluctuations first increases with time and then decreases to approach zero at large numbers of cells. In contrast, in classical size-independent proliferation models, where cell division occurs based on a pure timing mechanism, fluctuations in cell number increase monotonically over time to approach a nonzero value. We systematically study these differences and the convergence speed using different size control strategies.
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dc.identifierhttps://www.biorxiv.org/content/10.1101/2022.07.05.498901v1
dc.identifier2692-8205
dc.identifierhttp://hdl.handle.net/20.500.12324/39376
dc.identifierhttps://doi.org/10.1101/2022.07.05.498901
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/33704
dc.languageeng
dc.publisherCold Spring Harbor Laboratory
dc.relationBioRxiv
dc.relation1
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dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcebioRxiv Vol 6, (2022): bioRxiv Vol 6, (2022) P. 1-8.
dc.subjectGenética vegetal y fitomejoramiento - F30
dc.subjectEstructura celular
dc.subjectPropiedades biológicas
dc.subjectProliferación
dc.subjectTransversal
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_1414
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_1501
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_51dd9e80
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/cb9e98de-ab9f-4b9f-825c-a775c47771e1/download
dc.titleCell size regulation and proliferation fluctuations in single-cell derived colonies
dc.typeArtículo científico

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