Isolation and characterisation of aluminum tolerance genes in the cereals: an integrated functional genomic, molecular genetic and physiological analysis.
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Research activities and progress: sorghum and maize Al tolerance. Field testing in Africa. Tangible outputs. Research activities and progress Sorghum and maize Al tolerance. Sorghum. We have continued to characterise the major sorghum Al tolerance gene we cloned last year, Alt SB, which encodes a MATE transporter that mediates Al-activated root citrate exudation that is central to sorghum Al tolerance (Magalhães et al. 2007). In the course of developing NILs harboring different Alt SB alleles in the same genetic background to identify the best AltSB alleles for molecular breeding for improved sorghum Al tolerance, we have obtained clear evidence for several novel Al tolerance loci in sorghum that contribute to the significant transgressive segregation for Al tolerance that was observed (Caniato et al. 2007). Furthermore, we have found strong evidence that one or more of these novel tolerance genes probably interacts with Alt SB to facilitate maximal Alt SB expression and tolerance. We are currently producing highly Al tolerant lines useful for breeding, for our recently funded GCP grant on advancing sorghum Al tolerance.
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Genética molecular, Cereal, Alumínio, Sorgo, Milho
