Effect of a highly concentrated lipopeptide extract of Bacillus subtilis on fungal and bacterial cells.

Vista sencilla de ítem
dc.contributorAugusto Etchegaray, Faculdade de Química, PUC-Campinas
dc.contributorCarolina de Castro Bueno, Faculdade de Química, PUC-Campinas
dc.contributorITAMAR SOARES DE MELO, CNPMA
dc.contributorS. M. TSAI, CENA/USP
dc.contributorM. F. FIORE, CENA/USP
dc.contributorM. E. SILVA-STENICO, CENA/USP
dc.contributorL. A. B. de MORAES, Departamento de Química/USP
dc.contributorO. TESCHKE, Laboratório de Nanoestruturas e Interfaces, Instituto de Física, UNICAMP.
dc.creatorETCHEGARAY, A.
dc.creatorBUENO, C. de C.
dc.creatorMELO, I. S. de
dc.creatorTSAI, S. M.
dc.creatorFIORE, M. F.
dc.creatorSILVA-STENICO, M. E.
dc.creatorMORAES, L. A. B. de
dc.creatorTESCHKE, O.
dc.date2016-11-22T23:00:57Z
dc.date2016-11-22T23:00:57Z
dc.date2008-12-22
dc.date2008
dc.date2016-11-22T23:00:57Z
dc.date.accessioned2026-07-07T05:02:43Z
dc.descriptionLipopeptides produced by Bacillus subtilis are known for their high antifungal activity. The aim of this paper is to show that at high concentration they can damage the surface ultra-structure of bacterial cells. A lipopeptide extract containing iturin and surfactin (5 mg mL¡1) was prepared after isolation from B. subtilis (strain OG) by solid phase extraction. Analysis by atomic force microscope (AFM) showed that upon evaporation, lipopeptides form large aggregates (0.1?0.2 m2) on the substrates silicon and mica. When the same solution is incubated with fungi and bacteria and the system is allowed to evaporate, dramatic changes are observed on the cells. AFM micrographs show disintegration of the hyphae of Phomopsis phaseoli and the cell walls of Xanthomonas campestris and X. axonopodis. Collapses to fungal and bacterial cells may be a result of formation of pores triggered by micelles and lamellar structures, which are formed above the critical micelar concentration of lipopeptides. As observed for P. phaseoli, the process involves binding, solubilization, and formation of novel structures in which cell wall components are solubilized within lipopeptide vesicles. This is the Wrst report presenting evidences that vesicles of uncharged and negatively charged lipopeptides can alter the morphology of gramnegative bacteria
dc.identifierArchives of Microbiology, v. 190, n. 6, p.611-622, 2008.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/15579
dc.identifier.urihttp://hdl.handle.net/123456789/478395
dc.languageeng
dc.rightsopenAccess
dc.subjectFungo
dc.subjectControle biológico
dc.titleEffect of a highly concentrated lipopeptide extract of Bacillus subtilis on fungal and bacterial cells.
dc.typeArtigo de periódico

Archivos

Colecciones

Embrapa Medio Ambiente (CNPMA)