Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells

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dc.creatorMaheshwari, Chirag
dc.creatorCoe, Robert A.
dc.creatorKarki, Shanta
dc.creatorCovshoff, Sarah
dc.creatorTapia, Ronald
dc.creatorTyagi, Aruna
dc.creatorHibberd, Julian M.
dc.creatorFurbank, Robert T.
dc.creatorQuick, William Paul
dc.creatorLin, Hsiang-Chun
dc.date2021-05
dc.date2024-12-19T12:53:42Z
dc.date2024-12-19T12:53:42Z
dc.date.accessioned2026-06-27T04:11:35Z
dc.descriptionWe generated antisense constructs targeting two of the five Rubisco small subunit genes (OsRBCS2 and 4) which account for between 30–40 % of the RBCS transcript abundance in leaf blades. The constructs were driven by a maize phosphoenolpyruvate carboxylase (PEPC) promoter known to have enriched expression in mesophyll cells (MCs). In the resulting lines leaf, Rubisco protein content was reduced by between 30–50 % and CO2 assimilation rate was limited under photorespiratory and non-photorespiratory conditions. A relationship between Rubisco protein content and CO2 assimilation rate was found. This was associated with a significant reduction in dry biomass accumulation and grain yield of between 37–70%. In addition to serving as a resource for reducing Rubisco accumulation in a cell-preferential manner, these lines allow us to characterize gene function and isoform specific suppression on photosynthesis and growth. Our results suggest that the knockdown of multiple genes is required to completely reduce Rubisco accumulation in MCs.
dc.identifierhttps://hdl.handle.net/10568/164284
dc.identifier.urihttp://hdl.handle.net/123456789/24604
dc.languageen
dc.publisherElsevier
dc.rightsOpen Access
dc.sourceMaheshwari, Chirag; Coe, Robert A.; Karki, Shanta; Covshoff, Sarah; Tapia, Ronald; Tyagi, Aruna; Hibberd, Julian M.; Furbank, Robert T.; Quick, William Paul and Lin, Hsiang-Chun. 2021. Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells. Journal of Plant Physiology, Volume 260 p. 153395
dc.subjectagronomy and crop science
dc.subjectplant science
dc.subjectphysiology
dc.titleTargeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)