Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.].

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dc.contributorPAULO IVAN FERNANDES JUNIOR, CPATSA
dc.contributorANDRÉA APARECIDA DE LIMA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO
dc.contributorSAMUEL RIBEIRO PASSOS, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO
dc.contributorCARLOS ALBERTO TUAO GAVA, CPATSA
dc.contributorPAULO JANSEN DE OLIVEIRA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO
dc.contributorNORMA GOUVEA RUMJANEK, CNPAB
dc.contributorGUSTAVO RIBEIRO XAVIER, CNPAB.
dc.creatorFERNANDES JUNIOR, P. I.
dc.creatorLIMA, A. A. de
dc.creatorPASSOS, S. R.
dc.creatorGAVA, C. A. T.
dc.creatorOLIVEIRA, P. J. de
dc.creatorRUMJANEK, N. G.
dc.creatorXAVIER, G. R.
dc.date2015-04-10T05:42:19Z
dc.date2015-04-10T05:42:19Z
dc.date2013-02-22
dc.date2012
dc.date2015-04-10T05:42:19Z
dc.date.accessioned2026-07-07T05:43:10Z
dc.descriptionThis study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization
dc.identifierBrazilian Journal of Microbiology, v. 43, n. 4, p. 1604-1612, 2012.
dc.identifier1517-8382
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/950566
dc.identifier.urihttp://hdl.handle.net/123456789/498532
dc.languageeng
dc.rightsopenAccess
dc.subjectRizóbio
dc.subjectSeleção de estirpes
dc.subjectFixação biológica de nitrogênio
dc.subjectDiversidade fenotípica
dc.subjectFeijão
dc.subjectMelhoramento Vegetal
dc.subjectFeijão de Corda
dc.subjectBiotechnology
dc.subjectPigeon peas
dc.subjectMolecular genetics
dc.subjectNitrogen fixation
dc.titlePhenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.].
dc.typeArtigo de periódico

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