Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut.

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dc.contributorMARIA ISABEL G. MARTINS, RENORBIO/UFRPE; ANTONIO EUZÉBIO G. SANT'ANA, UFAL; FELIPE MATHEUS T. VASCONCELOS, UFRPE; WBYRATAN L. SILVA, UFAL; LIZIANE MARIA DE LIMA, CNPA; REGINALDO CARVALHO, UFRPE; PÉRICLES A. MELO FILHO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA.
dc.creatorMARTINS, M. I. G.
dc.creatorSANT'ANA, A. E. G.
dc.creatorVASCONCELOS, F. M. T.
dc.creatorSILVA, W. L.
dc.creatorLIMA, L. M. de
dc.creatorCARVALHO, R.
dc.creatorMELO FILHO, P. A.
dc.creatorSANTOS, R. C. dos
dc.date2017-03-03T23:57:51Z
dc.date2017-03-03T23:57:51Z
dc.date2017-03-03
dc.date2016
dc.date2017-03-03T23:57:51Z
dc.date.accessioned2026-06-30T23:15:41Z
dc.descriptionEssential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut.
dc.identifierAfrican Journal of Biotechnology, v. 15, n. 30, p. 1597-1607, July 2016.
dc.identifier1684-5315
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1065905
dc.identifier.urihttp://hdl.handle.net/123456789/377635
dc.languageeng
dc.rightsopenAccess
dc.subjectOcimum Basicilum L
dc.subjectAranha ácaro
dc.subjectSpider mites
dc.subjectAmendoim
dc.subjectTetranychus urticae
dc.subjectCromatografia
dc.subjectPeanuts
dc.subjectChromatography
dc.subjectEugenol
dc.titleBioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut.
dc.typeArtigo de periódico

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