In vitro propagation from nodal segments of Lippia origanoides (chemotype A)

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dc.creatorRamírez Moreno, Julián Sebastián
dc.creatorVega Porras, Sergio Andrés
dc.creatorEscobar Pérez, Roosevelt Humberto
dc.creatorStashenko, Elena E.
dc.creatorFuentes Lorenzo, Jorge Luis
dc.creatorGarzon Gutierrez, Luz Nayibe
dc.date2022-07
dc.date2023-02-20T14:38:00Z
dc.date2023-02-20T14:38:00Z
dc.date.accessioned2026-06-27T13:30:21Z
dc.descriptionThis research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/128769
dc.identifier.urihttp://hdl.handle.net/123456789/61507
dc.languageen
dc.publisherFapUNIFESP
dc.rightsOpen Access
dc.sourceRamírez Moreno, J.S.; Vega Porras, S.A.; Escobar, R.H.; Stashenko, E.E.; Fuentes Lorenzo, J.L.; Garzón Gutiérrez, L.N. (2022) In vitro propagation from nodal segments of Lippia origanoides (chemotype A). Ciência Rural, 52(7): e20210323. ISSN: 1678-4596
dc.subjectmicropropagation
dc.subjectshoots
dc.subjectnodes
dc.subjectprotocols
dc.subjectlippia
dc.subjectplant propagation
dc.titleIn vitro propagation from nodal segments of Lippia origanoides (chemotype A)
dc.typeJournal Article

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