EVALUACIÓN DE LA ESTABILIDAD GENÉTICA MEDIANTE MARCADORES RAPD EN PLANTAS DE Ipomoea batatas
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Instituto Nacional de Ciencias Agrícolas
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Donating plants coming from tuberous root sprouts, belonging to a sweet potato (Ipomoea batatas (L.) Lam.) clone, INIVIT B 93-1, were placed in flasks containing water under the semicontrolled conditions of a laboratory to induce bud break, as well as vitroplants coming from the somatic embryos of leaf limb calluses; in both cases, leaf limbs were used to perform the molecular studies of Random Amplification Polymorphic DNA (RAPD), to identify the material and evaluate the genetic stability of plants obtained from somatic embryos. The total genomic DNA was extracted from the material mentioned before, through a homogeneous mixture of leaf limb tissue (1,0 g) and liquid nitrogen. DNA concentration was determined by means of spectrophotometry and materials were evaluated for 10 arbitrary primers, following Operon Technologies¿ protocol recommendations, which were: OPF-15, OPF-14, OPA-13, OPF-13, OPF-04, OPF-07, OPF-01, OPF-03, OPA-12 and OPF-05. PCR products were electrophoretically separated at 1.5 % agarose gel in a TBE buffer, and they were stained with etidium bromide before being observed on an UV transiluminator. The amplification patterns obtained were compared by evaluating bands, both for its presence (1) and absence (0) per each of the treatments, then observing a complete monomorphism in the donating material bands, compared to the one coming from somatic embryo vitroplants.
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