High-frequency regeneration of plants in vitro from seedling-derived apical bud explants in Tilia mandshurica
| dc.coverage | Asia | |
| dc.coverage | Republic of Korea | |
| dc.creator | Kim, T. D., Lee, N. N., Kwon, E. K., Kim, Y. W. | |
| dc.date | 2023-04-27T13:58:01Z | |
| dc.date | 2023-04-27T13:58:01Z | |
| dc.date | 2022 | |
| dc.date | 2022-10-24T13:35:00.0000000Z | |
| dc.date.accessioned | 2026-06-28T00:58:24Z | |
| dc.description | <i>Tilia</i> species have taken hold as a crop of interest for both the honey and timber industries, underscoring a need for the development of efficient methods for large-scale propagation. In <i>Tilia</i> species such as <i>T. mandshurica</i>, propagation through seeds is difficult because of a low germination rate (approximately 10-16%) and an extremely hard seed coating that can delay germination for up to two years. Further, cutting propagation is not efficient due to the resulting poor development of root systems. To overcome these difficulties, <i>in vitro</i> culture techniques for plant regeneration have become attractive methods for the propagation of plants. Generally, plant regeneration systems based on apical and axillary bud culture are the most effective method of <i>in vitro</i> propagation<br /> This work was to develop an efficient method for shoot induction and plant regeneration of seedling-derived apical bud explant in <i>Tilia mandshurica</i>. The highest rate (82.2%) of shoot induction was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on MS medium containing 1.0 mg/L BAP. However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. For rooting of <i>in vitro</i>-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5-1.0 mg/L IBA or 3.0 mg/L NAA. In the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) or root length (18.5 cm). This work showed that a low concentration (0.5 mg/L) of IBA is appropriate for rooting. Keywords: Biodiversity conservation ID: 3622528 | |
| dc.format | 1p. | |
| dc.format | application/pdf | |
| dc.identifier | https://openknowledge.fao.org/handle/20.500.14283/cc2634en | |
| dc.identifier | http://www.fao.org/3/cc2634en/cc2634en.pdf | |
| dc.identifier.uri | http://hdl.handle.net/123456789/324027 | |
| dc.language | English | |
| dc.publisher | FAO ; | |
| dc.rights | Non-FAO | |
| dc.title | High-frequency regeneration of plants in vitro from seedling-derived apical bud explants in Tilia mandshurica | |
| dc.title | XV World Forestry Congress, 2-6 May 2022 | |
| dc.type | Document |