OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice

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dc.creatorLi, Jinjie
dc.creatorLi, Yang
dc.creatorYin, Zhigang
dc.creatorJiang, Jihong
dc.creatorZhang, Minghui
dc.creatorGuo, Xiao
dc.creatorYe, Zhujia
dc.creatorZhao, Yan
dc.creatorXiong, Haiyan
dc.creatorZhang, Zhanying
dc.creatorShao, Yujie
dc.creatorJiang, Conghui
dc.creatorZhang, Hongliang
dc.creatorAn, Gynheung
dc.creatorPaek, Nam-Chon
dc.creatorAli, Jauhar
dc.creatorLi, Zichao
dc.date2017-02
dc.date2024-12-19T12:54:48Z
dc.date2024-12-19T12:54:48Z
dc.date.accessioned2026-06-27T04:10:04Z
dc.descriptionDrought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of abscisic acid, stress and ripening (ASR) genes from upland rice variety, IRAT109 (Oryza sativa L. ssp. japonica), and demonstrated that overexpression of OsASR5 enhanced osmotic tolerance in Escherichia coli and drought tolerance in Arabidopsis and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of OsASR5 in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H2O2, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss‐of‐function mutant, osasr5, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone‐like protein and interacted with stress‐related HSP40 and 2OG‐Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that OsASR5 plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone‐like protein that possibly prevents drought stress‐related proteins from inactivation.
dc.identifierhttps://hdl.handle.net/10568/165181
dc.identifier.urihttp://hdl.handle.net/123456789/23737
dc.languageen
dc.publisherWiley
dc.rightsOpen Access
dc.sourceLi, Jinjie; Li, Yang; Yin, Zhigang; Jiang, Jihong; Zhang, Minghui; Guo, Xiao; Ye, Zhujia; Zhao, Yan; Xiong, Haiyan; Zhang, Zhanying; Shao, Yujie; Jiang, Conghui; Zhang, Hongliang; An, Gynheung; Paek, Nam‐Chon; Ali, Jauhar and Li, Zichao. 2017. OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice. Plant Biotechnology Journal, Volume 15 no. 2 p. 183-196
dc.titleOsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)