Induced spawning and larval rearing of grouper (Epinephelus salmoides Maxwell)
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Broodfish of grouper (Epinephelus salmoides) were induced to spawn by hormonal induction. The hormones used for this experiment were HCG + pituitary gland (PG) and LRH-a. The results showed that at the dosages of 500 IU HCG + 3 mg of PG per kg of fish for first injection and 1,000 IU HCG + 3 mg of PG per kg of fish at the final injection at an interval of 24 hours, the treated fish spawned naturally in a spawning tank 12 hours after the final injection. At lower dosages of 500 IU HCG + 3 mg PG a t 12-hour intervals or 500 IU HCG + 3 mg PG at 24-hour intervals or using 10 mg LRH-a at 12-hour intervals, the eggs can be artificially fertilized only by stripping. * This paper was presented at the First Asian Fisheries Forum 24–30 May, 1986 Manila, Philippines. ** Present Address : FAO/UNDP Seafarming Development Project (INS/81/008), FAO, Jakarta, Indonesia. The larval rearing experiment ws conducted in a 250-liter fiberglass tank at the stocking density of 2,500 larvae per tank. Feed s used in this experiment were : (a) Isochrysis + sea urchin eggs; (b) Isochrysis + Brachionus; and (c) Tetraselmis + Brachionus. The experiment results showed that newly hatched larvae fed with Isochrysis mixed with sea urchin eggs and then switched to Brachionus on day 10 gave the best survival rate (9% at day 30).