Molecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH).

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dc.contributorFERNANDO HERCOS VALICENTE, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS.
dc.creatorVALICENTE, F. H.
dc.creatorLANA, U. G. de P.
dc.date2011-04-10T11:11:11Z
dc.date2011-04-10T11:11:11Z
dc.date2009-08-10
dc.date2008
dc.date2016-03-15T11:11:11Z
dc.date.accessioned2026-07-07T05:04:31Z
dc.descriptionBacillus thuringiensis (Berliner) strains 344 and 1644 belonging to the CNPMS/Embrapa (Maize and Sorghum National Research Center) Microorganism Bank, located in Sete Lagoas, MG, showed to be highly efficient in controlling fall armyworm, Spodotpera frugiperda (J.E. Smith). Strains 344 and 1644 were isolated from soil samples originated from Iguassu Falls and Rolândia, respectively, Paraná State, Brazil. While strain 344 harbors cry1Ab, cry1B, cry1E and cry1Fb genes, strain 1644 showed cry1B, cry1C, and cry1D and cry1Fb genes. The strain 344 crystal has bipyramidal shape and showed LC50 equal to 8.21 x 106 spores/mL. Strain 1644 has cuboid crystal and showed LC50 equal to 2.07 x 106 spores/mL. Comparative analysis of protein profile, plasmid and amplification with primers ERIC allowed the differentiation of the evaluated strains.
dc.identifierRevista Brasileira de Milho e Sorgo, Sete Lagoas, v. 7, n. 3, p. 195-209, 2008.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/491685
dc.identifierhttp://dx.doi.org/10.18512/1980-6477/rbms.v7n3p195-209
dc.identifier.urihttp://hdl.handle.net/123456789/479454
dc.languageeng
dc.rightsopenAccess
dc.subjectCry genes
dc.subjectBacillus Thuringiensis
dc.subjectinsect pathology
dc.titleMolecular characterization of the Bacillus thuringiensis (BERLINER) strains 344 and 1644, efficient against fall armyworm Spodoptera frugiperda (J. E. SMITH).
dc.typeArtigo de periódico

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