Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

Dávia   Guimarães Pompeu; Marcelo Augusto  Mattioli; Rosy Iara   Maciel de Azambuja Ribeiro; Daniel   Bonoto Gonçalves; Juliana   Teixeira de Magalhães; Sérgio  Marangoni; José Antônio   da Silva; Paulo Afonso  Granjeiro

Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

Autores

Dávia Guimarães Pompeu

Marcelo Augusto Mattioli

Rosy Iara Maciel de Azambuja Ribeiro

Daniel Bonoto Gonçalves

Juliana Teixeira de Magalhães

Sérgio Marangoni

José Antônio da Silva

Paulo Afonso Granjeiro

Editor

Sociedade Brasileira de Ciência e Tecnologia de Alimentos

Descripción

A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool.