Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines

Vista sencilla de ítem
dc.creatorEmmrich, Peter M.F.
dc.creatorRejzek, Martin
dc.creatorHill, Lionel
dc.creatorBrett, Paul
dc.creatorEdwards, Anne
dc.creatorSarkar, Abhimanyu
dc.creatorField, Rob A.
dc.creatorMartin, Cathie
dc.creatorWang, Trevor L.
dc.date2019-12
dc.date2023-03-10T14:41:21Z
dc.date2023-03-10T14:41:21Z
dc.date.accessioned2026-06-27T17:02:00Z
dc.descriptionGrass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties.
dc.identifierhttps://hdl.handle.net/10568/129608
dc.identifier.urihttp://hdl.handle.net/123456789/140268
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceEmmrich, Peter M.F.; Rejzek, Martin; Hill, Lionel; Brett, Paul; Edwards, Anne; Sarkar, Abhimanyu; Field, Rob A.; Martin, Cathie; Wang, Trevor L. 2019. Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines. BMC Plant Biology 19:
dc.subjectidentification
dc.subjectpermits
dc.subjectpea
dc.titleLinking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines
dc.typeJournal Article

Archivos

Colecciones

Instituto Internacional de Investigaciones Pecuarias (ILRI)