Bacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers

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dc.creatorAlcantara, Edwin P.
dc.creatorAguda, Remedios M.
dc.creatorCurtiss, April
dc.creatorDean, Donald H.
dc.creatorCohen, Michael B.
dc.date2004-04
dc.date2024-12-19T12:56:41Z
dc.date2024-12-19T12:56:41Z
dc.date.accessioned2026-06-27T04:16:03Z
dc.descriptionThe receptor binding step in the molecular mode of action of five δ‐endotoxins (Cry1Ab, Cry1Ac, Cry1C, Cry2A, and Cry9C) from Bacillus thuringiensis was examined to find toxins with different receptor sites in the midgut of the striped stem borer (SSB) Chilo suppressalis (Walker) and yellow stem borer (YSB) Scirpophaga incertulas (Walker) (Lepidoptera: Pyralidae). Homologous competition assays were used to estimate binding affinities (Kcom) of 125I‐labelled toxins to brush border membrane vesicles (BBMV). The SSB BBMV affinities in decreasing order was: Cry1Ab = Cry1Ac > Cry9C > Cry2A > Cry1C. In YSB, the order of decreasing affinities was: Cry1Ac > Cry1Ab > Cry9C = Cry2A > Cry1C. The number of binding sites (Bmax) estimated by homologous competition binding among the Cry toxins did not affect toxin binding affinity (Kcom) to both insect midgut BBMVs. Results of the heterologous competition binding assays suggest that Cry1Ab and Cry1Ac compete for the same binding sites in SSB and YSB. Other toxins bind with weak (Cry1C, Cry2A) or no affinity (Cry9C) to Cry1Ab and Cry1Ac binding sites in both species. Cry2A had the lowest toxicity to 10‐day‐old SSB and Cry1Ab and Cry1Ac were the most toxic. Taken together, the results of this study show that Cry1Ab or Cry1Ac could be combined with either Cry1C, Cry2A, or Cry9C for more durable resistance in transgenic rice. Cry1Ab should not be used together with Cry1Ac because a mutation in one receptor site could diminish binding of both toxins. Arch. Insect Biochem. Physiol. 55:169–177, 2004. © 2004 Wiley‐Liss, Inc.
dc.identifierhttps://hdl.handle.net/10568/166803
dc.identifier.urihttp://hdl.handle.net/123456789/27109
dc.languageen
dc.publisherWiley
dc.sourceAlcantara, Edwin P.; Aguda, Remedios M.; Curtiss, April; Dean, Donald H. and Cohen, Michael B. 2004. Bacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers. Arch Insect Biochem Physiol, Volume 55 no. 4 p. 169-177
dc.subjectbacillus thuringiensis
dc.subjecttransgenic rice
dc.subjectchilo suppressalis
dc.subjectscirpophaga incertulas
dc.subjectendotoxins
dc.titleBacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)