Rice germin-like proteins: allelic diversity and relationships to early stress responses

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dc.creatorDavidson, Rebecca M.
dc.creatorManosalva, Patricia M.
dc.creatorSnelling, Jacob
dc.creatorBruce, Myron
dc.creatorLeung, Hei
dc.creatorLeach, Jan E.
dc.date2010-03
dc.date2024-12-19T12:55:48Z
dc.date2024-12-19T12:55:48Z
dc.date.accessioned2026-06-27T04:12:06Z
dc.descriptionGermin-like protein (GLP) markers were associated with quantitative trait loci (QTL) for resistance to the rice blast pathogen, Magnaporthe oryzae in multiple rice (Oryza sativa) mapping populations. Twelve paralogous OsGLP gene family members are located within the physical QTL region on chromosome 8, and gene silencing studies suggest that they contribute collectively to the resistance phenotype. We compared sequence and expression profiles of OsGLP alleles in two resistant and two susceptible parental rice lines to find functional polymorphisms that correlated with the resistant phenotype. Based on coding and promoter sequences, the genes belong to two germin subfamily groups (GER3 and GER4). OsGLP members from both subfamilies were constitutively expressed and developmentally regulated in all cultivars. Transient induction above constitutive levels was observed for some OsGLPs, especially GER4 subfamily members, at early time points after M. oryzae infection and mechanical wounding. Varying 5′ regulatory regions and differential expression of some family members between resistant and susceptible cultivars corresponded with differential hydrogen peroxide (H2O2) accumulation after the same stimuli. OsGLP of both GER subfamilies localized to the plant cell wall. The protein location and early gene induction suggest that OsGLPs protect rice leaves at early stages of infection before fungal penetration and subsequent ingress. Our data suggest that regulation of OsGLP genes defines resistant versus susceptible phenotypes.
dc.identifierhttps://hdl.handle.net/10568/166074
dc.identifier.urihttp://hdl.handle.net/123456789/24898
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceDavidson, Rebecca M.; Manosalva, Patricia M.; Snelling, Jacob; Bruce, Myron; Leung, Hei and Leach, Jan E. 2010. Rice germin-like proteins: allelic diversity and relationships to early stress responses. Rice, Volume 3 no. 1 p. 43-55
dc.subjectalleles
dc.subjectdisease resistance
dc.subjectfungal diseases
dc.subjectgenes
dc.subjectgene expression
dc.subjectgenetic diversity
dc.subjectgenetic mapping
dc.subjectnucleotide sequences
dc.subjectquantitative trait loci
dc.subjectplant proteins
dc.subjectpromoters
dc.subjectstress
dc.subjectstress response
dc.subjectmagnaporthe oryzae
dc.titleRice germin-like proteins: allelic diversity and relationships to early stress responses
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)