Direct detection of Mycobacterium sp in bovine and bubaline tissues by PCR.

ARAUJO, C. P.; OSORIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; FILHO, A. F. S.; VIDAL, C. E. S.; ROXO, E.; ROCHA, A.; FONSECA JUNIOR, A. A.; SILVA, M. R.; ARAUJO, F. R.

Direct detection of Mycobacterium sp in bovine and bubaline tissues by PCR.

Autores

FONSECA JUNIOR, A. A.

SILVA, M. R.

Descripción

In this study, a two-step PCR system was developed to detect Mycobacterium tuberculosis complex or Mycobacterium bovis directly from cattle tissues. The first reaction was conventional PCR, followed by a second real-time PCR with internal primers and 6FAM/MGBNFQ probe, both reactions for rv2807 (M. tuberculosis complex) or mmps6 (M. bovis).