Molecular diagnosis optimization of virus, bacteria and fungi in sugarcane.

Vista sencilla de ítem
dc.contributorHAIKO ENOK SAWAZAKI, IAC; LUIZ ALEXANDRE NOGUEIRA DE SA, CNPMA; CASSIARA REGINA NOVENTA CORREA BARBOSA GONÇALVES, CTC; RENATO FERRAS DE ARRUDA VEIGA, IAC; CARLOS AUGUSTO COLOMBO, IAC.
dc.creatorSAWAZAKI, H. E.
dc.creatorSA, L. A. N. de
dc.creatorGONÇALVES, C. R. N. C. B.
dc.creatorVEIGA, R. F. A.
dc.creatorCOLOMBO, C. A.
dc.date2013-08-01T11:11:11Z
dc.date2013-08-01T11:11:11Z
dc.date2013-08-01
dc.date2013
dc.date2013-08-02T11:11:11Z
dc.date.accessioned2026-07-07T04:56:20Z
dc.descriptionAbstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of primers designed for orange rust was more specific. The pair of primers designed for scald, apparently was not able to distinguish only bacteria of the genus Erwinia, whereas the primers described in the literature were observed amplify others genera, such as Xanthomonas, Pseudomonas, Erwinia, Stenotrophomona and Pantoea. The three strains of the fungus curvularia, found in sugarcane, resembled more to the species C.cymbopogonis, C.geniculata, C.sp. Of the 28 primer pairs tested, the best were shown for each of the disease-causing agent.
dc.identifierInternacional Research Journal of Plant Science, Sapele, v. 4, n. 3, p. 76-83, 2013.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/963146
dc.identifier.urihttp://hdl.handle.net/123456789/475065
dc.languageeng
dc.rightsopenAccess
dc.subjectLeaf scald
dc.subjectPCR
dc.subjectCana de açúcar
dc.subjectDoença de planta
dc.subjectDiagnóstico
dc.subjectBactéria
dc.subjectVírus
dc.subjectFungo
dc.subjectEscaldadura
dc.subjectFerrugem alaranjada
dc.subjectPlant diseases and disorders
dc.subjectSugarcane
dc.subjectRatoon stunting disease
dc.subjectScald diseases
dc.subjectSugarcane yellow leaf virus
dc.subjectSugarcane streak virus
dc.subjectFijivirus
dc.subjectSmut diseases
dc.subjectRust diseases
dc.subjectCurvularia
dc.subjectPolymerase chain reaction
dc.titleMolecular diagnosis optimization of virus, bacteria and fungi in sugarcane.
dc.typeArtigo de periódico

Archivos

Colecciones

Embrapa Medio Ambiente (CNPMA)