Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.

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dc.contributorTHOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.
dc.creatorFAJARDO, T. V. M.
dc.creatorNICKEL, O.
dc.date2014-11-03T11:11:11Z
dc.date2014-11-03T11:11:11Z
dc.date2014-11-03
dc.date2014
dc.date2014-11-03T11:11:11Z
dc.date.accessioned2026-07-07T11:22:06Z
dc.descriptionThe viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.
dc.identifierCiência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/999019
dc.identifier.urihttp://hdl.handle.net/123456789/505289
dc.languageeng
dc.rightsopenAccess
dc.subjectASGV
dc.subjectACLSV
dc.subjectASPV
dc.subjectApMV
dc.subjectMacieira
dc.subjectPolissonda
dc.subjectMaçã
dc.subjectDoença de planta
dc.subjectHibridação
dc.subjectApple stem grooving virus
dc.subjectApple chlorotic leaf spot virus
dc.subjectApple mosaic virus
dc.subjectApple stem pitting virus
dc.titleSimultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
dc.typeArtigo de periódico

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