Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)

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dc.creatorWoo, Mi-Ok
dc.creatorHam, Tae-Ho
dc.creatorJi, Hyeon-So
dc.creatorChoi, Min-Seon
dc.creatorJiang, Wenzhu
dc.creatorChu, Sang-Ho
dc.creatorPiao, Rihua
dc.creatorChin, Joong Hyoun
dc.creatorKim, Jung-A
dc.creatorPark, Bong Soo
dc.creatorSeo, Hak Soo
dc.creatorJwa, Nam-Soo
dc.creatorMcCouch, Susan
dc.creatorKoh, Hee-Jong
dc.date2008-04
dc.date2024-12-19T12:56:11Z
dc.date2024-12-19T12:56:11Z
dc.date.accessioned2026-06-27T04:10:43Z
dc.descriptionA rice genic male‐sterility gene ms‐h is recessive and has a pleiotropic effect on the chalky endosperm. After fine mapping, nucleotide sequencing analysis of the ms‐h gene revealed a single nucleotide substitution at the 3′‐splice junction of the 14th intron of the UDP‐glucose pyrophosphorylase 1 (UGPase1; EC2.7.7.9) gene, which causes the expression of two mature transcripts with abnormal sizes caused by the aberrant splicing. An in vitro functional assay showed that both proteins encoded by the two abnormal transcripts have no UGPase activity. The suppression of UGPase by the introduction of a UGPase1‐RNAi construct in wild‐type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms‐h mutant phenotype, whereas overexpression of UGPase1 in ms‐h mutant plants restored male fertility and the transformants produced T1 seeds that segregated into normal and chalky endosperms. In addition, both phenotypes were co‐segregated with the UGPase1 transgene in segregating T1 plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm. Our results suggest that UGPase1 plays a key role in pollen development as well as seed carbohydrate metabolism.
dc.identifierhttps://hdl.handle.net/10568/166377
dc.identifier.urihttp://hdl.handle.net/123456789/24095
dc.languageen
dc.publisherWiley
dc.sourceWoo, Mi‐Ok; Ham, Tae‐Ho; Ji, Hyeon‐So; Choi, Min‐Seon; Jiang, Wenzhu; Chu, Sang‐Ho; Piao, Rihua; Chin, Joong‐Hyoun; Kim, Jung‐A; Park, Bong Soo; Seo, Hak Soo; Jwa, Nam‐Soo; McCouch, Susan and Koh, Hee‐Jong. 2008. Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.). The Plant Journal, Volume 54 no. 2 p. 190-204
dc.subjectendosperm
dc.subjectgenes
dc.subjectgene expression
dc.subjectgene silencing
dc.subjectmale sterility
dc.subjectrna interference
dc.titleInactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)