Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii
| dc.creator | Akem, C. | |
| dc.creator | Dashiell, Kenton E. | |
| dc.date | 1991-08 | |
| dc.date | 2019-06-25T17:09:07Z | |
| dc.date | 2019-06-25T17:09:07Z | |
| dc.date.accessioned | 2026-06-27T16:06:46Z | |
| dc.description | Shoot-tips 15cm long from 15 soybean cultivars and breeding lines were individually immersed in Hoagland's solution in 1 × 14 cm test tubes, and supported by cotton plugs. All leaves were removed leaving about 1 cm of each petiole on the shoot. A 4 mm mycelial plug of Sclerotium rolfsii Sacc., taken from the periphery of a 3-day-old culture grown on acidified potato dextrose agar (PDA) media was placed between the stem and a petiole in the middle of the shoot. Tubes with shoots were then placed in a polyethylene enclosure in a growth room where the day and night temperatures averaged 31 ± 2°C and 24 ± 2°C, respectively. Relative humidity (r.h.) was maintained at 80–90% by lining the bottom of the enclosure with wet burlap. Lesions appeared on shoot tips 2 days after inoculation, and their length was measured 2, 3 and 4 days later. On three cultivars, TG × 1436-1 E, TG × 1596-2E and TG × 1614-1E, the rate of lesion expansion was significantly less than that on the other cultivars. One week after inoculation, tubes were drained, and shoots left in the chamber at 50–60% r.h. to allow sclerotia to form. Sclerotia from each shoot were collected. The cultivars TG × 1436-1 D and TG × 1596-2E produced the fewest sclerotia per shoot-tip. Sclerotial viability, determined by germination on PDA at 25 ± 2°C in darkness, ranged from 38 to 99%. This method is effective in differentiating reactions of soybean cultivars to S. rolfsii. | |
| dc.identifier | https://hdl.handle.net/10568/101772 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/122879 | |
| dc.language | en | |
| dc.publisher | Elsevier | |
| dc.rights | Limited Access | |
| dc.subject | soybeans | |
| dc.subject | breeding | |
| dc.subject | leaves | |
| dc.subject | petioles | |
| dc.subject | potatoes | |
| dc.subject | inoculation | |
| dc.subject | shoots | |
| dc.title | Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii | |
| dc.type | Journal Article |