Quantitative analysis of the intestinal bacterial communities in broiler chickens using qPCR and metagenomic analysis.
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The 16S rDNA sequence analysis has been one of the most used approaches for microbiota taxonomic profiling. Among the available techniques, real-time quantitative PCR (qPCR) and next generation sequencing (NGS) and metagenomic analysis has been widely used by researchers, because in addition to qualitative, it also provide quantitative data. Thus, the aim of our study was to compare the effectiveness of these 2 methods for quantification of 3 groups of bacteria of the intestinal microbiota of broilers fed on a standard corn-soybean based diet.
X-MEETING 2012.
X-MEETING 2012.
Palabras clave
Análise de DNA, Análise metagenômica, Metagenomics, Enterobacteriaceae