Expression of apomixis genes during reproductive development in Urochloa decumbens and Urochloa ruziziensis (Poaceae).
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This study aimed to correlate the developmental stages of the embryo sac with the morphometric traits of spikelets and to characterize the expression of the BbrizRan, BbrizSti1, and BbrizAGL6genes in Urochloa ruziziensis(sexual) and U. decumbens(apomictic). Data on the length and diameter of spikelets and anatomical sections were also obtained. Gene expression was determined using RT-qPCR in spikelets at megasporogenesis and megagametogenesis. The spikelet length could be used as a reliable morphological marker to discriminate between the two stages in both species. The embryo sacs observed were of the Polygonumtype in U. ruziziensisand the Panicumtype in U. decumbens. RT-qPCR analysis revealed that the BbrizRan, BbrizSti1, and BbrizAGL6genes were differentially expressed in the studied species during reproductive development. The BbrizSti1gene was more highly expressed in U. decumbens(apomictic) than in U. ruziziensis(sexual) during megasporogenesis, while for BbrizRan, U. decumbensshowed higher expression than U. ruziziensisduring both megasporogenesis and megagametogenesis. An increase in the expression level of BbrizAGL6was observed in both species at both developmental stages ofthe embryo sac.
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Expressão gênica, Megasporogênese, Megagametogênese, Braquiaria, Brachiaria, Apomixia, Reprodução Assexuada, Gramínea Forrageira