Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures

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dc.creatorKitani, H.
dc.creatorBlack, Samuel J.
dc.creatorNakamura, Y.
dc.creatorNaessens, Jan
dc.creatorMurphy, N.
dc.creatorYokomizo, Y.
dc.creatorGibson, John P.
dc.creatorIraqi, F.A.
dc.date2002-04
dc.date2010-05-17T06:53:59Z
dc.date2010-05-17T06:53:59Z
dc.date.accessioned2026-06-27T17:06:32Z
dc.descriptionMice whose tumor necrosis factor alpha (TNF-) genes were disrupted developed higher levels of parasitemia than wild-type mice following infection with Trypanosoma congolense IL1180 or T. brucei brucei GUTat3.1, confirming the results of earlier studies. To determine whether TNF- directly affects the growth of these and other bloodstream forms of African trypanosomes, we studied the effects of recombinant mouse, human, and bovine TNF- on the growth of two isolates of T. congolense, IL1180 and IL3338, and two isolates of T. brucei brucei, GUTat3.1 and ILTat1.1, under axenic culture conditions. The preparations of recombinant TNF- used were biologically active as determined by their capacity to kill L929 cells. Of five recombinant TNF- lots tested, one lot of mouse TNF- inhibited the growth of both isolates of T. brucei brucei and one lot of bovine TNF- inhibited the growth of T. brucei brucei ILTat1.1 but only at very high concentrations and without causing detectable killing of the parasites. The other lots of mouse recombinant TNF-, as well as human TNF-, did not affect the growth of any of the test trypanosomes even at maximal concentrations that could be attained in the culture systems (3,000 to 15,000 U of TNF-/ml of medium). These results suggest that exogenously added recombinant TNF- generally does not inhibit the growth of African trypanosomes under the culture conditions we used. The impact of TNF- on trypanosome parasitemia may be indirect, at least with respect to the four strains of trypanosomes reported here.
dc.identifierhttps://hdl.handle.net/10568/1540
dc.identifier.urihttp://hdl.handle.net/123456789/142431
dc.languageen
dc.publisherAmerican Society for Microbiology
dc.rightsLimited Access
dc.sourceKitani, H.; Black, S.J.; Nakamura, Y.; Naessens, J.; Murphy, N.; Yokomizo, Y.; Gibson, J.; Iraqi, F. 2002. Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures. Infection and Immunology 70(4):2210-2214.
dc.subjecttrypanosoma congolense
dc.subjectanimal diseases
dc.titleRecombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures
dc.typeJournal Article

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