Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation

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dc.creatorRamu, P.
dc.creatorEsuma, W.
dc.creatorKawuki, R.S.
dc.creatorRabbi, Ismail Y.
dc.creatorEgesi, Chiedozie N.
dc.creatorBredeson, J.V.
dc.creatorBart, R.S.
dc.creatorVerma, J.
dc.creatorBuckler, E.S.
dc.creatorLu, F
dc.date2017-06
dc.date2017-05-15T09:35:21Z
dc.date2017-05-15T09:35:21Z
dc.date.accessioned2026-06-27T16:07:11Z
dc.descriptionCassava (Manihot esculenta Crantz) is an important staple food crop in Africa and South America; however, ubiquitous deleterious mutations may severely decrease its fitness. To evaluate these deleterious mutations, we constructed a cassava haplotype map through deep sequencing 241 diverse accessions and identified >28 million segregating variants. We found that (i) although domestication has modified starch and ketone metabolism pathways to allow for human consumption, the concomitant bottleneck and clonal propagation have resulted in a large proportion of fixed deleterious amino acid changes, increased the number of deleterious alleles by 26%, and shifted the mutational burden toward common variants; (ii) deleterious mutations have been ineffectively purged, owing to limited recombination in the cassava genome; (iii) recent breeding efforts have maintained yield by masking the most damaging recessive mutations in the heterozygous state but have been unable to purge the mutation burden; such purging should be a key target in future cassava breeding.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/81024
dc.identifier.urihttp://hdl.handle.net/123456789/123074
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceRamu, P., Esuma, W., Kawuki, R., Rabbi, I.Y., Egesi, C., Bredeson, J.V., ... & Lu, F. (2017). Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation. Nature Genetics.
dc.subjectgenomics
dc.subjectplant genetics
dc.subjectpopulation genetics
dc.subjectcassava
dc.subjectdeleterious mutations
dc.subjecthaplotype map
dc.subjectclonal propagation
dc.titleCassava haplotype map highlights fixation of deleterious mutations during clonal propagation
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)