A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction
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For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate University. The RNA isolated using this standardized protocol was high in quality and led to successful good quality cDNA library construction and identification of functional ESTs in yam.
Palabras clave
yams, genotypes, colletotrichum gloeosporioides, cdna, rna, diseases