Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification

K.V  Ravishankar; R  Vasudeva; B  Hemanth; P  Nischita; B.R  Sthapit; V.A  Parthasarathy; V.R  Rao

Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification

Autores

Editor

Society for Promotion of Horticulture

Descripción

Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium sized evergreen tree found in western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by over exploitation and loss of habitat. Development of microsatellite markers would help in understanding genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. Probability of identity values ranged from 0.00329 to 0.30489. Cross species amplification SSR primers in the related species, showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta).