Genomics-based diagnostic marker development for Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola.

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dc.creatorLang, Jillian M.
dc.creatorHamilton, John P.
dc.creatorDiaz, Maria Genaleen Q.
dc.creatorVan Sluys, Marie Anne
dc.creatorBurgos, Ma. Ruby G.
dc.creatorVera Cruz, Casiana M.
dc.creatorBuell, C. Robin
dc.creatorTisserat, Ned A.
dc.creatorLeach, Jan E.
dc.date2010-03
dc.date2024-12-19T12:55:48Z
dc.date2024-12-19T12:55:48Z
dc.date.accessioned2026-06-27T04:09:40Z
dc.descriptionA computational genomics pipeline was used to compare sequenced genomes of Xanthomonas spp. and to rapidly identify unique regions for development of highly specific diagnostic markers. A suite of diagnostic primers was selected to monitor diverse loci and to distinguish the rice bacterial blight and bacterial leaf streak pathogens, Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola, respectively. A subset of these primers was combined into a multiplex polymerase chain reaction set that accurately distinguished the two rice pathogens in a survey of a geographically diverse collection of X. oryzae pv. oryzae, X. oryzae pv. oryzicola, other xanthomonads, and several genera of plant-pathogenic and plant- or seed-associated bacteria. This computational approach for identification of unique loci through whole-genome comparisons is a powerful tool that can be applied to other plant pathogens to expedite development of diagnostic primers.
dc.identifierhttps://hdl.handle.net/10568/166072
dc.identifier.urihttp://hdl.handle.net/123456789/23524
dc.languageen
dc.publisherScientific Societies
dc.sourceLang, J.M., Hamilton, J.P., Diaz, M.G.Q., Van Sluys, M.A., Burgos, Ma. R.G., Vera Cruz, C.M., Buell, C.R., Tisserat, N.A. and Leach, J.E. 2010. Genomics-based diagnostic marker development for Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola. Plant Disease, Volume 94 no. 3 p. 311-319
dc.subjectdiagnostic techniques
dc.subjectgenetic markers
dc.subjectgenome analysis
dc.subjectgenomes
dc.subjectgenomics
dc.subjectnucleotide sequences
dc.subjectplant pathogenic bacteria
dc.subjectpolymerase chain reaction
dc.subjectxanthomonas oryzae pv
dc.subjectoryzae
dc.subjectoryzicola
dc.titleGenomics-based diagnostic marker development for Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola.
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)