Identification of Eimeria mitis and Eimeria praecox in Broiler Feces Using Polymerase
Chain Reaction

MV  Meireles; LO  Roberto; RF  Riera

Identification of Eimeria mitis and Eimeria praecox in Broiler Feces Using Polymerase Chain Reaction

Autores

MV Meireles

LO Roberto

RF Riera

Editor

Fundação APINCO de Ciência e Tecnologia Avícolas

Descripción

There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA extraction using DNAzol followed by PCR can be a useful tool in epidemiological studies, since it provides fast and reliable detection of Eimeria sp. in field samples.