Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda

dc.creatorKugonza, Donald R.
dc.creatorHan Jianlin
dc.creatorOkeyo Mwai, Ally
dc.creatorKiwuwa, G.H.
dc.creatorHanotte, Olivier H.
dc.date2006
dc.date2011-09-03T18:40:45Z
dc.date2011-09-03T18:40:45Z
dc.date.accessioned2026-06-27T16:52:06Z
dc.descriptionNineteen microsatellite markers were used to investigate the genetic diversity, phylogenetic relationships and herd structure of Ugandan Ankole cattle. A total of 304 animals from 8 herds in Mbarara District were characterised. Across all loci, 200 alleles were observed. A high mean number of alleles (MNA) per locus, ranging from 5.89 to 6.79 per herd, was observed. Polymorphic information content (PIC) ranged from 0.403 (ILSTS013) to 0.817 (ILSTS036), with an overall mean of 0.688. The average observed heterozygosity (Ho) was highest in Kaibanda (0.727) and lowest in Kituuha (0.648), while the expected heterozygosity (He) ranged from 0.722 (Nshaara) to 0.664 (Kituuha). Significant deviations from Hardy-Weinberg proportions were observed on 8 loci, however, all 152 loci-herd combination tests were in equilibrium after Bonferroni correction. FST estimates for all loci and between all herds were highly significant (P<0.001), suggesting little if any gene flow between the herds. For all herd pairs, FST values were generally low, with an overall mean of 0.041 + 0.08. Significant (P<0.01) inbreeding effect (FIS) was detected in the Nasasira herd. The mean number of migrants per generation (Nm) across all herds was 3.82. Phylogenetic analysis showed that herds from the same geographical counties grouped together. These results illustrate at the molecular level the fairly wide genetic variation found among the Ankole herds studied and therefore the potential for genetic improvement of these herds.
dc.identifierhttps://hdl.handle.net/10568/5523
dc.identifier.urihttp://hdl.handle.net/123456789/135559
dc.languageen
dc.publisherTSAP and ILRI
dc.rightsOpen Access
dc.sourceKugonza, D.R., Han Jianlin, Okeyo, A.M., Kiwuwa, G.H. and Hanotte, O. 2006. Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda. In: Rege, J.E.O.; Nyamu, A.M.; Sendalo, D. (eds.). 2006. The role of biotechnology in animal agriculture to address poverty in Africa: Opportunities and challenges. Proceedings of the 4th All Africa Conference on Animal Agriculture and the 31st annual meeting of Tanzania Society for Animal Production, Arusha, Tanzania, 20-24 September 2005. Dar es Salaam, Tanzania: TSAP and Nairobi, Kenya: ILRI.
dc.titleMicrosatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda
dc.typeConference Paper

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