Primers annealing to the coding sequences can identify SH3 gene variants.

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dc.contributorPAULA CRISTINA DA SILVA ANGELO, CNPCA
dc.contributorMICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA
dc.contributorLUCIANA H. SHIGUEOKA, IDR - PARANÁ
dc.contributorGUSTAVO H. SERA, IDR - PARANÁ
dc.contributorEVELINE TEIXEIRA CAIXETA MOURA, CNPCA.
dc.creatorANGELO, P. C. da S.
dc.creatorYAMAGISHI, M. E. B.
dc.creatorSHIGUEOKA, L. H.
dc.creatorSERA, G. H.
dc.creatorCAIXETA, E. T.
dc.date2025-11-06T20:49:04Z
dc.date2025-11-06T20:49:04Z
dc.date2025-11-06
dc.date2025
dc.date.accessioned2026-07-01T00:27:34Z
dc.descriptionSH3 resistance factor to the coffee leaf rust was found in C. liberica and has been valued because it is durable. Working in silico, we demonstrated that SH3 loci are complex in most species displaying four up to eight SH3-NBS LRR gene variants placed in chromosomes 3. One additional variant can be found isolated in chromosomes 6. We recently proposed structural models for six C. liberica SH3-Rx-CCNBS-LRR variants [1]. Among these, three are very similar to each other and seems exclusive to C. liberica. This work was directed to develop a primer system useful to identify those three variants (LIB2, 3 and 4), and demonstrate its presence/absence in genotypes with introgression from C. liberica.
dc.formatp. 82.
dc.identifierIn: CONFERENCE OF ASSOCIATION FOR THE SCIENCE AND INFORMATION ON COFFEE, 30., 2025, Lisboa. Book of abstracts.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1181262
dc.identifier.urihttp://hdl.handle.net/123456789/404560
dc.languageeng
dc.rightsopenAccess
dc.subjectGene SH3-NBS
dc.subjectLoci SH3
dc.subjectCoffea liberica
dc.subjectDNA primers
dc.titlePrimers annealing to the coding sequences can identify SH3 gene variants.
dc.typeResumo em anais e proceedings

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