Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil.

Vista sencilla de ítem
dc.contributorÉRICO DE C. DIANESE, UNIVERSIDADE DE BRASÍLIA
dc.contributorEDUARDO D. RAMALHO, UNIVERSIDADE DE BRASÍLIA
dc.contributorDANIELA M. CERQUEIRA, CERQUEIRA, D. M.
dc.contributorDANIELA BIAGGIONI LOPES, CPATSA
dc.contributorTHOR VINICIUS MARTINS FAJARDO, CNPUV
dc.contributorMARISA A. S. V. FERREIRA, UNIVERSIDADE DE BRASÍLIA
dc.contributorCLÁUDIA R. F. MARTINS, UNIVERSIDADE DE BRASÍLIA.
dc.creatorDIANESE, E. de C.
dc.creatorRAMALHO, E. D.
dc.creatorCERQUEIRA, D. M.
dc.creatorLOPES, D. B.
dc.creatorFAJARDO, T. V. M.
dc.creatorFERREIRA, M. A. S. V.
dc.creatorMARTINS, C. R. F.
dc.date2018-10-06T00:40:00Z
dc.date2018-10-06T00:40:00Z
dc.date2005-03-03
dc.date2005
dc.date2018-10-06T00:40:00Z
dc.date.accessioned2026-07-07T05:51:41Z
dc.descriptionMany viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). Acomplex of eight viruses [Grapevine leafroll-associated virus (GLRaV)-1 to 8] is associated with this disease. The objectiveof this study was to compare the variability of the 3? terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase.
dc.identifierFitopatologia Brasileira, v. 30, n. 2, p. 173-176, mar./abr. 2005.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/155711
dc.identifier.urihttp://hdl.handle.net/123456789/501399
dc.languageeng
dc.rightsopenAccess
dc.subjectVideira
dc.subjectPolimerase
dc.subjectGLRa-3
dc.subjectVitis spp
dc.subjectVale do São francisco
dc.subjectDoença
dc.subjectRNA
dc.subjectUva
dc.subjectVírus
dc.subjectAmpelovirus
dc.titleVariability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil.
dc.typeArtigo de periódico

Archivos

Colecciones

Embrapa Semi-Árido (CPATSA)