Association of β-globin polymorphisms and tolerance to haemonchosis in ewes and lambs of different sheep breeds.

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dc.contributorRAFAELA TAMI IKEDA KAPRITCHKOFF, Universidade Estadual Paulista; CINTIA HIROMI OKINO, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; HORNBLENDA JOAQUINA SILVA BELLO; RENATA SILVA MATOS; GLAUCIA ROBERTA MELITO; FLAVIA ALINE BRESSANI DONATONI, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE.
dc.creatorKAPRITCHKOFF, R. T. I.
dc.creatorOKINO, C. H.
dc.creatorNICIURA, S. C. M.
dc.creatorBELLO, H. J. S.
dc.creatorMATOS, R. S.
dc.creatorMELITO, G. R.
dc.creatorDONATONI, F. A. B.
dc.creatorESTEVES, S. N.
dc.creatorCHAGAS, A. C. de S.
dc.date2024-03-22T20:32:21Z
dc.date2024-03-22T20:32:21Z
dc.date2024-03-21
dc.date2024
dc.date.accessioned2026-07-07T05:33:26Z
dc.descriptionGastrointestinal nematodes (GIN), especially Haemonchus contortus, represent a significant challenge for sheep production. Given the global concern about GIN anthelmintic resistance, alternative control methods able to reduce the dependence on these drugs are highly advisable. Since previous studies have shown that sheep carrying the Hb-A allele of β-globin are more resistant to H. contortus, this study aimed to investigate the relationship between the different haplotypes (Hb-AA, Hb-AB and Hb-BB) and phenotypes in Santa Inês (SI), Texel (TX) and White Dorper (DO) breeds infected with H. contortus. Blood samples were collected from 180 ewes and 123 lambs of the three breeds for DNA extraction followed by qPCR using a hydrolysis probe to identify the β-globin haplotypes. Phenotypic data, including fecal egg count (FEC), packed cell volume (PCV), FAMACHA score and body condition score for ewes and lambs, as well as weight gain for lambs, were collected. The genotypic frequencies of β-globin for ewes and lambs were, respectively: 21.7% and 21.4% Hb-AA, 50% and 50% Hb-AB and 28.3% and 28.6% Hb-BB in SI; 0% and 0% Hb-AA, 18.6% and 9.4% Hb-AB and 81.4% and 90.6% Hb-BB in TX; and 0% and 0% Hb-AA, 13.1% and 0% Hb-AB and 86.9% and 100% Hb-BB in DO. In ewes, mean PCV differed (p<0.05) between the three haplotypes, with higher PCV in Hb-AA animals, followed by Hb-AB and Hb-BB. When considering each breed separately, SI Hb-AA ewes presented higher PCV (p<0.05), highlighting that even in a breed already considered resistant, animals with Hb-AA haplotype showed superior performance. Lambs with the Hb-AA haplotype exhibited a higher (p<0.05) mean PCV compared to those with Hb-AB and Hb-BB. The same pattern was found in SI when analyzing each breed separately. No significant association was found between β-globin haplotypes and FEC, FAMACHA score, body condition score, or weight gain. Nevertheless, given that anemia is the major clinical sign of haemonchosis, our findings on PCV reinforce that sheep carrying the Hb-A allele of β-globin are more tolerant to haemonchosis. This study may support the development of a valuable tool, targeting genetic selection for GIN control, reducing the dependence on anthelmintics and boosting sheep production worldwide.
dc.format8 p.
dc.identifierVeterinary Parasitology, v. 328, june 2024, 110163.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1163085
dc.identifier10.1016/j.vetpar.2024.110163
dc.identifier.urihttp://hdl.handle.net/123456789/494897
dc.languageeng
dc.rightsopenAccess
dc.subjectParasite tolerance
dc.subjectOvine
dc.subjectResilience
dc.subjectHaemonchus Contortus
dc.subjectAnemia
dc.subjectHaplotypes
dc.subjectGenotyping
dc.titleAssociation of β-globin polymorphisms and tolerance to haemonchosis in ewes and lambs of different sheep breeds.
dc.typeArtigo de periódico

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