TEMPERATURE AND SUBSTRATE INFLUENCES ON SEED GERMINATION AND SEEDLING FORMATION IN Callisthene fasciculata Mart. (VOCHYSIACEAE) IN THE LABORATORY
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Universidade Federal de Viçosa
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ABSTRACT – Callisthene fasciculata Mart. is a tree belonging to the Vochysiaceae family. Its wood is moderatelyheavy and resistant and used to make poles, beams, and other structures. The aim of this work was to evaluateseed germination and the initial growth of seedlings of C. fasciculataat different temperatures and in differentsubstrates. Seeds were collected from fruits in the Pantanal de Miranda, Mato Grosso do Sul state, Brazil.In one experiment, the seeds were subjected to constant temperatures of 20, 25, 30 and 35 °C and to alternatingtemperatures of 20-30 and 25-35 °C (on paper substrate). In another experiment, the seeds were subjectedto temperatures of 20 and 25 °C on three substrates (sand, vermiculite and between paper) in a germinator.The experiment had a randomized design, with four replicates of 25 seeds per treatment. The F-values obtainedfor germination indicated no significant effect of substrate or temperature on the final germination percentage.The analyses revealed no effect of a treatment interaction (temperature x substrate) on either germinationor average germination time; however, a treatment interaction effect was observed on the germination speedindex. The treatment combinations yielding the best performance were between paper substrate at 20 °C andsand substrate at 25 °C. There was a significant effect of the interaction between temperature and substrateon seedling growth, with increased root growth observed in the between paper substrate at 25 °C and increasedaerial component growth in both sand at 20 °C and vermiculite at 25 °C. The between paper treatment at25 °C yielded the greatest final seedling size. Between paper is the most recommended substrate for the productionof seedlings due to its ease of handling and lower probability of contamination.
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