Agrobacterium tumefaciens–mediated genome editing in banana

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dc.creatorTripathi, J.
dc.creatorTripathi, L.
dc.date2025
dc.date2025-12-02T13:05:39Z
dc.date2025-12-02T13:05:39Z
dc.date.accessioned2026-06-27T16:08:48Z
dc.descriptionThis protocol outlines the Agrobacterium tumefaciens–mediated transformation process for genome editing in banana (Musa spp.). As a crop of significant agricultural and economic importance globally, improving banana cultivars is crucial for addressing challenges such as disease resistance, climate resilience and yield enhancement. The procedure presented here involves the establishment of embryogenic cell suspensions (ECSs) from immature male flowers, followed by co-cultivation with Agrobacterium containing the desired gene construct. Key steps include callus induction, ECS generation and maintenance, transformation using plasmid construct containing genome editing reagents such as CRISPR/Cas9, and regeneration. The method ensures the editing of target genes in the banana genome, facilitating genetic improvements.
dc.identifierhttps://hdl.handle.net/10568/178440
dc.identifier.urihttp://hdl.handle.net/123456789/123795
dc.languageen
dc.publisherHumana Press
dc.rightsLimited Access
dc.sourceTripathi, J.N. & Tripathi, L. (2025). Agrobacterium tumefaciens–mediated genome editing in banana. In C. Klein (eds) Agrobacterium. Methods in molecular biology, vol 2911. New York, USA: Humana, (p. 143-153).
dc.subjectbananas
dc.subjectmusa
dc.subjectflowers
dc.subjectagrobacterium tumefaciens
dc.subjectgene editing
dc.titleAgrobacterium tumefaciens–mediated genome editing in banana
dc.typeBook Chapter

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Instituto Internacional de Agricultura Tropical (IITA)