Increased transient genetic transformation in immature embryos of Brazilian BR 451 maize co-cultivated with Agrobacterium tumefaciens

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dc.creatorDielli Aparecida Didoné
dc.creatorMarilia Rodrigues de Silva
dc.creatorCássia Canzi Ceccon
dc.creatorTiago Teixeira
dc.creatorMarilei Suzin
dc.creatorMagali Ferrari Grando
dc.date2018
dc.date.accessioned2026-07-07T03:52:56Z
dc.descriptionGenetic engineering has amplified the possibilities of crop breeding and supported sustainability ideals. Agrobacterium tumefaciens is the preferred transformation system, since it produces transgenic plants with more stable transgene expression and inheritance. The agrobacteria and plant tissue must be co-cultivated in conditions that allow gene transfer. This study aimed to evaluate how co-cultivation time and temperature affect the transformation of immature maize embryos of the Hi-II hybrid (model genotype) and the Brazilian BR 451 variety with A. tumefaciens EHA101:pTF102. The pTF102 plasmid carries a uidA reporter gene that enables transient transformation to be quickly verified by GUS histochemical assays. Increasing the co-cultivation period from three to five days at 20°C resulted in a higher number of GUS positive embryos and blue spots per embryo in the BR 451 Brazilian variety, indicating better bacterial T-DNA transfer into the target explant cells. This condition raised the BR 451 response level to match the response level of the Hi-II control genotype, indicating that this Brazilian variety is suitable for genetic transformation.
dc.formatapplication/pdf
dc.identifier1679-9275
dc.identifierhttps://www.redalyc.org/articulo.oa?id=303058204041
dc.identifierhttps://www.redalyc.org/journal/3030/303058204041/
dc.identifierhttps://www.redalyc.org/journal/3030/303058204041/html/
dc.identifierhttps://www.redalyc.org/journal/3030/303058204041/303058204041.epub
dc.identifierhttps://www.redalyc.org/journal/3030/303058204041/movil
dc.identifier10.4025/actasciagron.v40i1.36475
dc.identifier.urihttp://hdl.handle.net/123456789/445976
dc.languageen
dc.publisherUniversidade Estadual de Maringá
dc.relationhttp://www.redalyc.org/revista.oa?id=3030
dc.rightsActa Scientiarum. Agronomy
dc.sourceActa Scientiarum. Agronomy (Brasil) Vol.40
dc.subjectAgrociencias
dc.titleIncreased transient genetic transformation in immature embryos of Brazilian BR 451 maize co-cultivated with Agrobacterium tumefaciens
dc.typeartículo científico

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Acta Scientiarum. Agronomy - Universidade Estadual de Maringá