Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses

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dc.creatorKreuze, Jan F.
dc.creatorPérez, A.
dc.creatorUntiveros, M.
dc.creatorQuispe, D.
dc.creatorFuentes, S.
dc.creatorBarker, I.
dc.creatorSimon, R.
dc.date2009-05
dc.date2017-02-07T13:03:33Z
dc.date2017-02-07T13:03:33Z
dc.date.accessioned2026-06-27T17:59:50Z
dc.descriptionWe report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assembly software and could positively identify RNA, ssDNA and dsDNA reverse transcribing viruses and in one case spanned the entire genome. The results present a novel approach which cannot only identify known viral pathogens, occurring at extremely low titers, but also novel viruses, without the necessity of any prior knowledge.
dc.identifierhttps://hdl.handle.net/10568/79776
dc.identifier.urihttp://hdl.handle.net/123456789/151906
dc.languageen
dc.publisherElsevier
dc.rightsLimited Access
dc.sourceKreuze, J.F.; Perez, A.; Untiveros, M.; Quispe, D.; Fuentes, S.; Barker, I.; Simon, R. 2009. Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses. Virology. (USA). ISSN 0042-6822. 388(1):1-7.
dc.subjectgenomes
dc.subjectviroses
dc.subjectdiagnosis
dc.subjectplant viruses
dc.subjectsweet potatoes
dc.titleComplete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses
dc.typeJournal Article

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Centro Internacional de la Papa (CIP)