Chromosome number and cytogenetics of Euphorbia heterophylla L.

dc.contributorJuliana Roriz Aarestrup, Bolsista da FAPEMIG; DECIO KARAM, CNPMS; Geraldo W. Fernandes, UFMG.
dc.creatorAARESTRUP, J. R.
dc.creatorKARAM, D.
dc.creatorFERNANDES, G. W.
dc.date2011-04-10T11:11:11Z
dc.date2011-04-10T11:11:11Z
dc.date2009-01-12
dc.date2008
dc.date2018-05-24T11:11:11Z
dc.date.accessioned2026-07-07T05:13:50Z
dc.descriptionEuphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome nwnber, morphology, and behavior of mitotic chromosomes. Seeds were germinated and submitted to four treatments to obtain metaphases: 0.5% co1chicine for 2 to 5 h, at ambient temperature; 0.5% co1chicine for 16 to 24 h; 0.0029 M 8-hydroxyquinoline (8-HQ) for 2 to 5 h at ambient temperature, and 0.0029 M 8-HQ for 16 to 24 h at 4 degrees C. The material was then fixed in methanol: acetic acid (3: I) and kept at -20 degrees C for 24 h. Roots were macerated in the enzyme solution ofFlaxzyme (TM) (NOVO FERMENT T)-distilled water (I: 40) at 34 degrees C for 2 h and later fixed again. Chromosome preparations were obtained by the dissociation of the apical meristems. The best chromosome preparations were obtained with the use of 8-HQ for 21 h 30 min at 4 degrees C. E. heterophylla showed 2n = 28 chromosomes. The short arm of the largest pair of chromosomes of the complement (pair nwnber 1) displayed a secondary constriction while the nuc1eolus was observed in the interphasic cell. Structural rearrangements were also observed in the E. heterophylla L. genome. The genomic instability associated with polyploidy may be the result of selection shaped by environmental adaptations and/or human-induced manipulation through agricultural practices.
dc.identifierGenetics and Molecular Research, Ribeirão Preto, v. 7, n. 1, p. 217-222, 2008.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/491644
dc.identifier.urihttp://hdl.handle.net/123456789/484223
dc.languageeng
dc.rightsopenAccess
dc.subjectPlanta daninha
dc.subjectevolution
dc.subjectpolyploidy
dc.titleChromosome number and cytogenetics of Euphorbia heterophylla L.
dc.typeArtigo de periódico

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