Evaluation of a Commercial ELISA Kit (IDEXX) to Differentiate AI Virus-infected Poultry from AI- Vaccinated Poultry (DIVA)

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dc.creatorF Talazadeh
dc.creatorM Mayahi
dc.creatorM Seifi
dc.creatorM Pourmehdi
dc.date2014
dc.date.accessioned2026-07-07T03:23:40Z
dc.descriptionVaccination programs for the control of avian influenza (AI) in poultry have limitations due to the problem of differentiating between vaccinated and virus-infected birds. The use of AI vaccination in poultry would have greater worldwide acceptance if reliable tests that clearly discriminated between naturally infected and vaccinated-only animals (DIVA) were available. In this study, to differentiate avian influenza virus (AIV)-infected chickens vs. chickens immunized with inactivated avian influenza virus, a commercial ELISA Kit (IDEXX) was used. Using HI as the reference method, the sensitivity of the commercial ELISA kit was 100% and its specificity was 100%. In the present study, we demonstrated that, 3 weeks after infection or vaccination, sera from both infected and vaccinated groups were tested positive and the difference in mean optical density between vaccinated and challenged birds, as detected by a commercial ELISA kit (IDEXX), was very small. Therefore, this assay cannot distinguish infected from vaccinated poultry.
dc.formatapplication/pdf
dc.identifier1516-635X
dc.identifierhttps://www.redalyc.org/articulo.oa?id=179731466010
dc.identifier.urihttp://hdl.handle.net/123456789/432590
dc.languageen
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relationhttp://www.redalyc.org/revista.oa?id=1797
dc.rightsRevista Brasileira de Ciência Avícola
dc.sourceRevista Brasileira de Ciência Avícola (Brasil) Num.2 Vol.16
dc.subjectAgrociencias
dc.titleEvaluation of a Commercial ELISA Kit (IDEXX) to Differentiate AI Virus-infected Poultry from AI- Vaccinated Poultry (DIVA)
dc.typeartículo científico

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Revista Brasileira de Ciência Avícola - Fundação APINCO de Ciência e Tecnologia Avícolas