Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.

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dc.contributorROSANGELA BEVITORI, CNPAF
dc.contributorMARILIA BARROS OLIVEIRA
dc.contributorMARIA FATIMA GROSSI DE SA, CENARGEN
dc.contributorANNA CRISTINA LANNA, CNPAF
dc.contributorRICARDO DIÓGENES DA SILVEIRA
dc.contributorSILVANA PETROFEZA, UFG.
dc.creatorBEVITORI, R.
dc.creatorOLIVEIRA, M. B.
dc.creatorGROSSI-DE-SÁ, M. F.
dc.creatorLANNA, A. C.
dc.creatorSILVEIRA, R. D. da
dc.creatorPETROFEZA, S.
dc.date2018-07-12T01:02:29Z
dc.date2018-07-12T01:02:29Z
dc.date2015-01-23
dc.date2014
dc.date2018-07-12T01:02:29Z
dc.date.accessioned2026-07-07T11:51:04Z
dc.descriptionDrought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.
dc.identifierGenetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1006581
dc.identifierhttp://dx.doi.org/10.4238/2014.November.27.7
dc.identifier.urihttp://hdl.handle.net/123456789/514970
dc.languageeng
dc.rightsopenAccess
dc.subjectArroz
dc.subjectOryza sativa
dc.subjectDoença de planta
dc.subjectBrusone
dc.subjectGenética molecular
dc.subjectSeca
dc.subjectGene expression
dc.subjectRice
dc.subjectMagnaporthe oryzae
dc.subjectBlast disease
dc.titleSelection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.
dc.typeArtigo de periódico

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