Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen

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dc.creatorMarín, M.L.
dc.creatorMafla, G.
dc.creatorRoca, W.
dc.creatorWithers, Lyndsey A.
dc.date1990
dc.date2017-08-28T12:45:48Z
dc.date2017-08-28T12:45:48Z
dc.date.accessioned2026-06-27T14:35:16Z
dc.descriptionCryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were obtained by both slow and rapid cooling methods followed by slow thawing (1 hour at -70 degrees C followed by 1 hour at -15 degrees C). In the case of whole seeds, slow thawing prevented shattering. Recovery of whole plants occurred from all surviving seeds but was reduced to 25-34 percent for control and frozen embryos. This drop is attributed to physical injury during dissection. (AS)
dc.identifierhttps://hdl.handle.net/10568/83253
dc.identifier.urihttp://hdl.handle.net/123456789/82489
dc.languageen
dc.rightsLimited Access
dc.sourceMarin, M.L.; Mafla, G.; Roca, W.M.; Withers, L.A. 1990. Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen. Cryo-Letters 11:257-264.
dc.subjectmanihot esculenta
dc.subjectcryopreservation
dc.subjectbiotechnology
dc.subjectseed
dc.subjecttemperature
dc.subjectgermination
dc.subjectwater content
dc.subjectstorage
dc.subjectcriopreservacion
dc.subjectbiotecnología
dc.subjectsemillas
dc.subjecttemperatura
dc.subjectgerminacion
dc.subjectcontenido de agua
dc.subjectalmacenamiento
dc.titleCryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen
dc.typeJournal Article

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Centro Internacional de Agricultura Tropical (CIAT)