Walnut SSR raw data, Central Asia

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dc.creatorGaisberger, Hannes
dc.creatorLegay, Sylvain
dc.date2020-05
dc.date2020-06-22T02:26:14Z
dc.date2020-06-22T02:26:14Z
dc.date.accessioned2026-06-27T13:27:32Z
dc.descriptionBetween September 2013 and September 2015, samples of Juglans regia L. were collected from three countries in Central Asia: Tajikistan, Uzbekistan, and Kyrgyzstan. We selected 360 sample individuals from wild tree population (four populations of 30 individuals in each country) and 270 samples from cultivated populations from home gardens (three populations of 30 individuals in each country). Within each wild population, a systematic sampling experimental design has been chosen to collect the 30 individual adult trees with at 50m regular intervals. From each sampled tree, three leaves were collected and stored in silica for subsequent genetic analyses conducted at the Luxembourg Institute of Science and Technology (LIST). Genomic DNA was extracted from ground silica dried leaf material using the NucleoSpin Plant II kit (Macherey-Nagel). All SSR loci were PCR amplified following Pollegioni et al. (2014) and multiplex PCR products analyzed on an ABI3500 Genetic Analyser (Life Technologies, Waltham, MA). Subsequently, fragment analysis was conducted using the Genemapper 5.0 software. ND: No Data after re-extraction and re-amplification of the sample.
dc.identifierhttps://hdl.handle.net/10568/108544
dc.identifier.urihttp://hdl.handle.net/123456789/60027
dc.languageen
dc.publisherBioversity International and the International Center for Tropical Agriculture
dc.relationhttps://hdl.handle.net/10568/108277
dc.rightsOpen Access
dc.sourceGaisberger, H.; Legay, S., 2020 Walnut SSR raw data, Central Asia, https://doi.org/10.7910/DVN/VUP7OH, Harvard Dataverse, V1, UNF:6:254awoJkmwuQGPFAPzG/Aw== [fileUNF]
dc.subjectclimate change
dc.subjectcambio climatico
dc.subjectgenetics
dc.subjectgenetica
dc.subjecttrees
dc.subjectarboles
dc.subjectjuglans regia
dc.titleWalnut SSR raw data, Central Asia
dc.typeDataset

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