A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
No hay miniatura disponible
Fecha
Título de la revista
ISSN de la revista
Título del volumen
Editor
Springer
Resumen
Descripción
Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre- and post-freezing steps. Refining the methodology led to a consistent 50-70 percent plant recovery. (RA)
Palabras clave
manihot esculenta, biological conservation, freezing, mandioca, conservación biológica, congelación