A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.

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dc.contributorANNA V. PROTASIO, Wellcome Trust Sanger Institute
dc.contributorISHENG J. TSAI, Wellcome Trust Sanger Institute
dc.contributorANNE BABBAGE, Wellcome Trust Sanger Institute
dc.contributorSARAH NICHOL, Wellcome Trust Sanger Institute
dc.contributorMARTIN HUNT, Wellcome Trust Sanger Institute
dc.contributorMARTIN A. ASLETT, Wellcome Trust Sanger Institute
dc.contributorNISHADI DE SILVA, Wellcome Trust Sanger Institute
dc.contributorGILES S. VELARDE, Wellcome Trust Sanger Institute
dc.contributorTIM J. C. ANDERSON, Texas Biomedical Research Institute
dc.contributorRICHARD C. CLARK, Wellcome Trust Sanger Institute
dc.contributorCLAIRE DAVIDSON, Wellcome Trust Sanger Institute
dc.contributorGARY P. DILLON, Wellcome Trust Sanger Institute
dc.contributorNANCY E. HOLROYD, Wellcome Trust Sanger Institute
dc.contributorPHILIP T. LOVERDE, University of Texas Health Science Center
dc.contributorCHRISTINE LLOYD, Wellcome Trust Sanger Institute
dc.contributorJACQUELLINE MCQUILLAN, Wellcome Trust Sanger Institute
dc.contributorGUILHERME OLIVEIRA, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz
dc.contributorTHOMAS D. OTTO, Wellcome Trust Sanger Institute
dc.contributorSOPHIA J. PARKER-MANUEL, University of York
dc.contributorMICHAEL A. QUAIL, Wellcome Trust Sanger Institute
dc.contributorR. ALAN WILSON, University of York
dc.contributorADHEMAR ZERLOTINI NETO, CNPTIA
dc.contributorDAVID W. DUNNE, University of Cambridge
dc.contributorMATTHEW BERRIMAN, Wellcome Trust Sanger Institute.
dc.creatorPROTASIO, A. V.
dc.creatorTSAI, I. J.
dc.creatorBABBAGE, A.
dc.creatorNICHOL, S.
dc.creatorHUNT, M.
dc.creatorASLETT, M. A.
dc.creatorSILVA, N. de
dc.creatorVELARDE, G. S.
dc.creatorANDERSON, T. J. C.
dc.creatorCLARK, R. C.
dc.creatorDAVIDSON, C.
dc.creatorDILLON, G. P.
dc.creatorHOLROYD, N. E.
dc.creatorLOVERDE, P. T.
dc.creatorLLOYD, C.
dc.creatorMCQUILLAN, J.
dc.creatorOLIVEIRA, G.
dc.creatorOTTO, T. D.
dc.creatorPARKER-MANUEL, S. J.
dc.creatorQUAIL, M. A.
dc.creatorWILSON, R. A.
dc.creatorZERLOTINI, A.
dc.creatorDUNNE, D. W.
dc.creatorBERRIMAN, M.
dc.date2018-05-11T00:37:25Z
dc.date2018-05-11T00:37:25Z
dc.date2012-11-21
dc.date2012
dc.date2020-04-14T11:11:11Z
dc.date.accessioned2026-07-07T04:15:36Z
dc.descriptionSchistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasite?s life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research.
dc.identifierPLOS Neglected Tropical Diseases, v. 6, n. 1, p. 1-13, Jan. 2012.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/940202
dc.identifier10.1371/journal.pntd.0001455
dc.identifier.urihttp://hdl.handle.net/123456789/455802
dc.languageeng
dc.rightsopenAccess
dc.subjectSchistosoma
dc.titleA systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.
dc.typeArtigo de periódico

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