Graphene oxide-silver nanoparticle hybrid material: an integrated nanosafety study in zebrafish embryos.

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dc.contributorALINE MARIA ZIGIOTTO DE MEDEIROS, CNPEM; LATIF ULLAH KHAN, CNPEM; GABRIELA HELENA DA SILVA, CNPEM; CARLOS ALBERTO OSPINA, CNPEM; OSWALDO LUIZ ALVES, IQ-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; DIEGO STÉFANI TEODORO MARTINEZ, CNPEM.
dc.creatorMEDEIROS, A. M. Z.
dc.creatorKHAN, L. U.
dc.creatorSILVA, G. H. da
dc.creatorOSPINA, C. A.
dc.creatorALVES, O. L.
dc.creatorCASTRO, V. L. S. S. de
dc.creatorMARTINEZ, D. S. T.
dc.date2021-04-05T15:33:57Z
dc.date2021-04-05T15:33:57Z
dc.date2021-04-05
dc.date2021
dc.date.accessioned2026-07-07T05:08:25Z
dc.descriptionAbstract: This work reports an integrated nanosafety study including the synthesis and characterization of the graphene oxide-silver nanoparticle hybrid material (GO-AgNPs) and its nano-ecotoxicity evaluation in the zebrafish embryo model. The influences of natural organic matter (NOM) and a chorion embryo membrane were considered in this study, looking towards more environmentally realistic scenarios and standardized nanotoxicity testing. The nanohybrid was successfully synthesized using the NaBH4 aqueous method, and AgNPs (~ 5.8 nm) were evenly distributed over the GO surface. GO-AgNPs showed a dose-response acute toxicity: the LC50 was 1.5 mg L-1 for chorionated embryos. The removal of chorion, however, increased this toxic effect by 50%. Furthermore, the presence of NOM mitigated mortality, and LC50 for GO-AgNPs changed respectively from 2.3 to 1.2 mg L-1 for chorionated and de-chorionated embryos. Raman spectroscopy confirmed the ingestion of GO by embryos; but without displaying acute toxicity up to 100 mg L-1, indicating that the silver drove toxicity down. Additionally, it was observed that silver nanoparticle dissolution has a minimal effect on these observed toxicity results. Finally, understanding the influence of chorion membranes and NOM is a critical step towards the standardization of testing for zebrafish embryo toxicity in safety assessments and regulatory issues.
dc.identifierEcotoxicology and Environmental Safety, v. 209, article 111776, 2021.
dc.identifier0147-6513
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1131052
dc.identifierhttps://doi.org/10.1016/j.ecoenv.2020.111776
dc.identifier.urihttp://hdl.handle.net/123456789/481723
dc.languageeng
dc.rightsopenAccess
dc.subjectNanohybrids
dc.subjectNanoecotoxicology
dc.subjectNanotoxicity
dc.subjectAlternative methods
dc.subjectGrafeno
dc.subjectNanomateriais
dc.subjectComposto de Carbono
dc.subjectToxidez
dc.subjectPeixe de Água Doce
dc.subjectEcotoxicology
dc.subjectGraphene oxide
dc.subjectNanomaterials
dc.subjectDanio rerio
dc.subjectchorion
dc.titleGraphene oxide-silver nanoparticle hybrid material: an integrated nanosafety study in zebrafish embryos.
dc.typeArtigo de periódico

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