A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens

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dc.creatorWorthington, Margaret
dc.creatorHeffelfinger, Christopher
dc.creatorBernal, Diana
dc.creatorQuintero, Constanza
dc.creatorZapata, Yeny Patricia
dc.creatorPérez, Juan Guillermo
dc.creatorVega, José de
dc.creatorMiles, John W.
dc.creatorDellaporta, Stephen L.
dc.creatorTohme, Joseph M.
dc.date2016-07-01
dc.date2016-07-26T22:10:19Z
dc.date2016-07-26T22:10:19Z
dc.date.accessioned2026-06-27T14:46:09Z
dc.descriptionApomixis, asexual reproduction through seed, enables breeders to identify and faithfully propagate superior heterozygous genotypes by seed without the disadvantages of vegetative propagation or the expense and complexity of hybrid seed production. The availability of new tools such as genotyping by sequencing and bioinformatics pipelines for species lacking reference genomes now makes the construction of dense maps possible in apomictic species, despite complications including polyploidy, multisomic inheritance, self-incompatibility, and high levels of heterozygosity. In this study, we developed saturated linkage maps for the maternal and paternal genomes of an interspecific Brachiaria ruziziensis (R. Germ. and C. M. Evrard) × B. decumbens Stapf. F1 mapping population in order to identify markers linked to apomixis. High-resolution molecular karyotyping and comparative genomics with Setaria italica (L.) P. Beauv provided conclusive evidence for segmental allopolyploidy in B. decumbens, with strong preferential pairing of homologs across the genome and multisomic segregation relatively more common in chromosome 8. The apospory-specific genomic region (ASGR) was mapped to a region of reduced recombination on B. decumbens chromosome 5. The Pennisetum squamulatum (L.) R.Br. PsASGR-BABY BOOM-like (psASGR–BBML)-specific primer pair p779/p780 was in perfect linkage with the ASGR in the F1 mapping population and diagnostic for reproductive mode in a diversity panel of known sexual and apomict Brachiaria (Trin.) Griseb. and P. maximum Jacq. germplasm accessions and cultivars. These findings indicate that ASGR–BBML gene sequences are highly conserved across the Paniceae and add further support for the postulation of the ASGR–BBML as candidate genes for the apomictic function of parthenogenesis.
dc.identifierhttps://hdl.handle.net/10568/76280
dc.identifier.urihttp://hdl.handle.net/123456789/87930
dc.languageen
dc.publisherOxford University Press
dc.rightsOpen Access
dc.sourceWorthington, Margaret; Heffelfinger, Christopher; Bernal, Diana; Quintero, Constanza; Zapata, Yeny Patricia; Perez, Juan Guillermo; De Vega, Jose; Miles, John; Dellaporta, Stephen; Tohme, Joseph M.. 2016. A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens. Genetics 203 (3): 1117-1132.
dc.subjectfeed crops
dc.subjectpoliploidy
dc.subjectgenetic markers
dc.subjectgenetic maps
dc.subjecthybridization
dc.subjectrestriction fragment
dc.subjectapomixis
dc.subjectgenotypes
dc.subjectbrachiaria decumbens
dc.subjectplant breeding
dc.subjectpoliploidia
dc.subjectmarcadores genéticos
dc.subjectmapas genéticos
dc.subjecthibridación
dc.subjectgenotipos
dc.titleA parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens
dc.typeJournal Article

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Centro Internacional de Agricultura Tropical (CIAT)