Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus Biopesticide.

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dc.contributorKARINA KLAFKE, UNIVERSIDADE SÃO PAULO; MARCIO MARTINELLO SANCHES, CNPGC; WILLIAM SIHLER, CENARGEN; MARLINDA LOBO DE SOUZA, CENARGEN; ALDO TONSO, UNIVERSIDADE DE SÃO PAULO.
dc.creatorKLAFKE, K.
dc.creatorSANCHES, M. M.
dc.creatorSIHLER, W.
dc.creatorSOUZA, M. L. de
dc.creatorTONSO, A.
dc.date2024-12-28T17:51:18Z
dc.date2024-12-28T17:51:18Z
dc.date2024-12-13
dc.date2024
dc.date.accessioned2026-07-07T03:42:25Z
dc.descriptionABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine- supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 1011 OBs/L.
dc.identifierIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA INDUSTRIAL, 1., 2024, Florianópolis. Creating connections between biotechnology and industrial sustainability: anais. Florianópolis: Universidade Federal de Santa Catarina, 2024.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1170651
dc.identifier.urihttp://hdl.handle.net/123456789/441313
dc.languageeng
dc.rightsopenAccess
dc.subjectSpodoptera Frugiperda
dc.subjectControle Biológico
dc.subjectBaculovirus
dc.subjectBiological control
dc.subjectBioreactors
dc.subjectBiopesticides
dc.titleBioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus Biopesticide.
dc.typeArtigo em anais e proceedings

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