Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis.

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dc.contributorPEDRO AVELLAR-COSTA
dc.contributorDANIELLE CABRAL DOS SANTOS
dc.contributorCARLA CHRISTINE LANGE, CNPGL
dc.contributorMARINELLA SILVA LAPORT
dc.contributorMARIA APARECIDA VASCONCELOS PAIVA BRITO
dc.contributorCIRO CÉSAR ROSSI
dc.contributorMARCIA GIAMBIAGI-DEMARVAL.
dc.creatorAVELLAR-COSTA, P.
dc.creatorSANTOS, D. C. DOS
dc.creatorLANGE, C. C.
dc.creatorLAPORT, M. S.
dc.creatorBRITO, M. A. V. P.
dc.creatorROSSI, C. C.
dc.creatorGIAMBIAGI-DEMARVAL, M.
dc.date2019-12-30T18:13:34Z
dc.date2019-12-30T18:13:34Z
dc.date2019-12-30
dc.date2019
dc.date2020-01-09T11:11:11Z
dc.date.accessioned2026-07-07T03:47:30Z
dc.descriptionABSTRACT - We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high accuracy of the MALDI-TOF results (97.7% accuracy, with only one strain misidentified) encourage the use of this technique, since it does not require laborious sample preparation, being fast and simple to perform. RESUMO - Nós comparamos o potencial de técnicas rotineiras utilizadas para a identificação de espécies de Staphylococcus, com o objetivo de avaliar a acurácia delas na detecção de 43 isolados de Staphylococcus chromogenes envolvidos com mastite bovina que, apesar de ser uma espécie coagulase-negativa, são capazes de coagular o plasma. Essas cepas poderiam ser erroneamente suspeitas de serem S. aureus e levarem a um tratamento não adequado da doença. MALDI-TOF, PCR-RFLP do gene da chaperonina groEL e sequenciamento do gene do rRNA 16S e do gene do fator de elongação Tu, tuf, foram avaliados. Os resultados dos quatro métodos foram coincidentes para apenas metade das cepas, devido à baixa precisão da PCR-RFLP com groEL (51,2% de acurácia). Apesar de todos os resultados do sequenciamento serem idênticos, a alta precisão dos resultados do MALDI-TOF (97,7% de acurácia, com apenas uma cepa identificada incorretamente) encoraja o uso dessa técnica, pois, não requer preparação laboriosa de amostras, sendo rápida e simples de executar.
dc.identifierCiência Rural, v. 49, n. 6, e20190168, 2019.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1117891
dc.identifierhttps://doi.org/10.1590/0103-8478cr20190168
dc.identifier.urihttp://hdl.handle.net/123456789/442982
dc.languageeng
dc.rightsopenAccess
dc.subjectMALDI-TOF
dc.subjectTuf
dc.subjectGroEL
dc.subjectStaphylococcus chromogenes
dc.subjectMastitis
dc.titleAccurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis.
dc.typeArtigo de periódico

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