Ocimum gratissimum L: A natural alternative against fungi associated with bean and maize seeds during storage

Vista sencilla de ítem
dc.creatorJuliana Trindade Lima
dc.creatorAntonio Fernando de Souza
dc.creatorHildegardo Seibert França
dc.date2022
dc.date.accessioned2026-07-07T02:43:33Z
dc.descriptionThe aim of the study was to evaluate in vitro antioxidant and antifungal activities of the ethanolic extract and its fractions from Ocimum gratissimum leaves. The ethanolic extract was obtained by maceration in ethanol and subsequent fractionation with solvents of increasing polarity (hexane, dichloro-methane, ethyl acetate and butanol). The Minimum Inhibitory Concentration (MIC) was determined for the ethanol extract and dichloromethane fraction. The antioxidant capacity was evaluated by DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) free radical scavenging methods, and by FRAP (Ferric Reducing Antioxidant Power). The in vitro antifungal effect was determined by the agar diffusion method on Aspergillus sp. and Rhizopus sp. fungi associated with corn and bean seeds during storage. The best samples with antifungal effect were determined by gas chromatography-mass spectrometry (GC/MS). The ethanolic extract had strong antioxidant capacity for all tested methods (DPPH 371.10±2.98 µg ml-1, ABTS 182.43±1.10 µg ml-1, FRAP 262.39±3.61 TEAC). Regarding the antifungal activity, the ethanolic extract and dichloromethane fraction resulted in total suppression (100%) of fungal growth and MIC ranged from 0.625 to 1.25 mg ml-1. In the GC/MS analysis, 22 substances were detected in all samples evaluated, with predominance of eugenol. These results indicated high biological potential of this plant as a biofungicide.
dc.formatapplication/pdf
dc.identifier0120-9965
dc.identifierhttps://www.redalyc.org/articulo.oa?id=180377650009
dc.identifierhttps://www.redalyc.org/journal/1803/180377650009/
dc.identifierhttps://www.redalyc.org/journal/1803/180377650009/html/
dc.identifierhttps://www.redalyc.org/journal/1803/180377650009/180377650009.epub
dc.identifierhttps://www.redalyc.org/journal/1803/180377650009/movil
dc.identifier10.15446/agron.colomb.v40n3.105851
dc.identifier.urihttp://hdl.handle.net/123456789/421947
dc.languageen
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1803
dc.rightsAgronomía Colombiana
dc.sourceAgronomía Colombiana (Colombia) Num.3 Vol.40
dc.subjectAgrociencias
dc.titleOcimum gratissimum L: A natural alternative against fungi associated with bean and maize seeds during storage
dc.typeartículo científico

Archivos

Colecciones

Agronomía Colombiana - Universidad Nacional de Colombia